Generator
Sir3
Part:BBa_K106695:Design
Designed by: Andrew Horwitz Group: iGEM08_UCSF (2008-10-28)
Sir3 under a strong constitutive promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2824
Illegal EcoRI site found at 4422
Illegal PstI site found at 2263 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2824
Illegal EcoRI site found at 4422
Illegal PstI site found at 2263
Illegal NotI site found at 4470 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2824
Illegal EcoRI site found at 4422
Illegal BglII site found at 1832
Illegal BglII site found at 2205
Illegal BglII site found at 2427
Illegal BamHI site found at 4453
Illegal XhoI site found at 4344 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2824
Illegal EcoRI site found at 4422
Illegal PstI site found at 2263 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2824
Illegal EcoRI site found at 4422
Illegal PstI site found at 2263
Illegal NgoMIV site found at 229
Illegal AgeI site found at 2950 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 879
Illegal BsaI.rc site found at 4161
Illegal SapI site found at 3061
Design Notes
Sir3 was cloned as an A!D ended part.
Source
Sir3 from genomic DNA.